Review





Similar Products

anti-green fluorescent protein (gfp) antibody  (Antibodies Inc)
99
Antibodies Inc anti-green fluorescent protein (gfp) antibody
Anti Green Fluorescent Protein (Gfp) Antibody, supplied by Antibodies Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/Anti-Green+Fluorescent+Protein+(GFP)+Antibody/custom%40gfp-1010%4042555355
Average 99 stars, based on 1 article reviews
anti-green fluorescent protein (gfp) antibody - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
αgfp hrp antibodies  (Miltenyi Biotec)
96
Miltenyi Biotec αgfp hrp antibodies
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
αgfp Hrp Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/GFP+Antibody/pmc13425104-245-7-9
Average 96 stars, based on 1 article reviews
αgfp hrp antibodies - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
primary antibody goat anti gfp  (Rockland Immunochemicals)
96
Rockland Immunochemicals primary antibody goat anti gfp
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Primary Antibody Goat Anti Gfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/ANTI-GREEN+FLUORESCENT+PROTEIN+(GFP%2C+RE/pmc12848578-176-14-18
Average 96 stars, based on 1 article reviews
primary antibody goat anti gfp - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
gfp hrp  (Miltenyi Biotec)
96
Miltenyi Biotec gfp hrp
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Gfp Hrp, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/GFP+Antibody/pmc13378056-18-0-2
Average 96 stars, based on 1 article reviews
gfp hrp - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti gfp magnetic beads  (Abbkine Inc)
86
Abbkine Inc anti gfp magnetic beads
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magnetic Beads, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/anti+gfp/pmc13254979-407-17-20
Average 86 stars, based on 1 article reviews
anti gfp magnetic beads - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
anti gfp magnetic ip kit  (Abbkine Inc)
86
Abbkine Inc anti gfp magnetic ip kit
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magnetic Ip Kit, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/anti+gst+ip+ipkinetm+kit+magnetic/pmc13254979-52-0-5
Average 86 stars, based on 1 article reviews
anti gfp magnetic ip kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
resource source identifier antibodies anti gfp hrp antibody miltenyi biotec  (Miltenyi Biotec)
96
Miltenyi Biotec resource source identifier antibodies anti gfp hrp antibody miltenyi biotec
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Resource Source Identifier Antibodies Anti Gfp Hrp Antibody Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/GFP+Antibody/pm42320466-157-2-8
Average 96 stars, based on 1 article reviews
resource source identifier antibodies anti gfp hrp antibody miltenyi biotec - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti gfp magbeads  (Yeasen Biotechnology)
86
Yeasen Biotechnology anti gfp magbeads
The pool of Cf-4 and <t>Cf-9</t> <t>proteins</t> interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with <t>GFP</t> beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.
Anti Gfp Magbeads, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+gfp+antibody/anti+ha+magbeads+mouse/pm42315065-101-0-2
Average 86 stars, based on 1 article reviews
anti gfp magbeads - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


The pool of Cf-4 and Cf-9 proteins interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with GFP beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.

Journal: Plant Physiology

Article Title: The C-terminal tail of Cf resistance proteins determines the intensity of the effector-triggered hypersensitive response-related cell death

doi: 10.1093/plphys/kiag476

Figure Lengend Snippet: The pool of Cf-4 and Cf-9 proteins interacting with SOBIR1 is larger than that of Cf-2 and Cf-5. (a ) Cf-4, Cf-9, Cf-2, and Cf-5, all tagged with eGFP, were co-expressed with Sl SOBIR1-Myc in leaves of N. benthamiana . After 2 dpi, total protein was extracted and incubated with GFP beads for immunoprecipitation. The immunoprecipitated proteins were subjected to immunoblotting with αGFP antibodies to visualize the eGFP-tagged Cf proteins and with αMyc antibodies to detect the Myc-tagged SOBIR1 protein. Rubisco bands indicate the total protein content of the input samples. b and c) Cf-4-eGFP, Cf-5-eGFP, and Cf-2-eGFP were transiently expressed in leaves of N. benthamiana , and at 2 dpi, total proteins were extracted and subjected to immunoprecipitation using GFP beads. Peptides were obtained from the immunoprecipitated proteins by a tryptic digest and were analyzed by LFQ nano-MS/MS. b) Comparison of the co-immunopurified proteins between Cf-4 and Cf-5, and c) between Cf-4 and Cf-2, by volcano plots. Significantly enriched proteins are indicated by the black dots. The light gray dots represent proteins that are not significantly enriched. Note that with Cf-4-eGFP, more SOBIR1 protein is purified than with Cf-5-eGFP or Cf-2-eGFP.

Article Snippet: Proteins tagged with eGFP were detected using αGFP-HRP antibodies (Miltenyi Biotec, 130-091-833), while αMyc antibodies (cMyc9E10, sc-40, Santa Cruz) were used to detect Myc-tagged proteins, followed by incubation with αMouse-HRP (GE Healthcare) as a secondary antibody.

Techniques: Incubation, Immunoprecipitation, Western Blot, Tandem Mass Spectroscopy, Comparison, Purification

The C-terminal tail of Cf proteins plays a role in the affinity of the Cf protein for SOBIR1. a) eGFP-tagged Cf-4 and Cf-5 and the various chimeric Cf proteins were transiently co-expressed with Myc-fused Sl SOBIR1 in leaves of N. benthamiana (OD 600 = 0.5). At 2 dpi, total protein was extracted and subjected to immunoprecipitation (IP) using GFP-trap beads, followed by western blotting with αGFP (upper panels) and αMyc (bottom panels) antibodies. b) Relative quantification of the amounts of Sl SOBIR1 protein co-purifying with Cf-4 and Cf-5 and with the chimeric Cf proteins. The relative amount of Sl SOBIR1 co-purifying with the wild-type Cf proteins was set to 1. An ANOVA/Dunnett's multiple comparison was used to determine statistical significance. Error bars display the SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Plant Physiology

Article Title: The C-terminal tail of Cf resistance proteins determines the intensity of the effector-triggered hypersensitive response-related cell death

doi: 10.1093/plphys/kiag476

Figure Lengend Snippet: The C-terminal tail of Cf proteins plays a role in the affinity of the Cf protein for SOBIR1. a) eGFP-tagged Cf-4 and Cf-5 and the various chimeric Cf proteins were transiently co-expressed with Myc-fused Sl SOBIR1 in leaves of N. benthamiana (OD 600 = 0.5). At 2 dpi, total protein was extracted and subjected to immunoprecipitation (IP) using GFP-trap beads, followed by western blotting with αGFP (upper panels) and αMyc (bottom panels) antibodies. b) Relative quantification of the amounts of Sl SOBIR1 protein co-purifying with Cf-4 and Cf-5 and with the chimeric Cf proteins. The relative amount of Sl SOBIR1 co-purifying with the wild-type Cf proteins was set to 1. An ANOVA/Dunnett's multiple comparison was used to determine statistical significance. Error bars display the SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Proteins tagged with eGFP were detected using αGFP-HRP antibodies (Miltenyi Biotec, 130-091-833), while αMyc antibodies (cMyc9E10, sc-40, Santa Cruz) were used to detect Myc-tagged proteins, followed by incubation with αMouse-HRP (GE Healthcare) as a secondary antibody.

Techniques: Immunoprecipitation, Western Blot, Quantitative Proteomics, Comparison